Fig. 4: FISH images of ANME-SRB consortia in seep sediment samples using oligonucleotide probes targeting ANME-2b (ANME-2b-726), ANME-2a (ANME-2a-828), or ANME-2c (ANME-2c-760); (in red), a SEEP-SRB1a (Seep1a-1441) probe (in yellow) and a newly-designed probe (Seep1g-1443) targeting the SEEP-SRB1g clade (in green) demonstrating physical association between ANME-2b and SEEP-SRB1g. | The ISME Journal

Fig. 4: FISH images of ANME-SRB consortia in seep sediment samples using oligonucleotide probes targeting ANME-2b (ANME-2b-726), ANME-2a (ANME-2a-828), or ANME-2c (ANME-2c-760); (in red), a SEEP-SRB1a (Seep1a-1441) probe (in yellow) and a newly-designed probe (Seep1g-1443) targeting the SEEP-SRB1g clade (in green) demonstrating physical association between ANME-2b and SEEP-SRB1g.

From: Experimentally-validated correlation analysis reveals new anaerobic methane oxidation partnerships with consortium-level heterogeneity in diazotrophy

Fig. 4

DAPI counterstain is shown in blue. Seep sediments harboring ANME-2a and ANME-2b (a–d) host ANME-SRB consortia that are composed of either ANME-2a–SEEP-SRB1a or ANME-2b–SEEP-SRB1g (b–d). FISH analysis of ANME-SRB consortia from sediments rich in ANME-2c and ANME-2b (e–h) documented ANME-SRB consisting of ANME-2b–SEEP-SRB1g or ANME-2c–SEEP-SRB1a partnerships (f–h); ANME-SRB consortia positively hybridized with the SEEP-SRB1g or SEEP-SRB1a probes were not observed to hybridize with probes targeting ANME-2c (h) or ANME-2b (e), respectively. In all panels, the scale bar is 10 µm.

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