Fig. 1: Serratia infections in native and non-native mosquito hosts.

Infection of CxSm1^RifR and CxSm2^SmR into Cx. quinquefasciatus (gray) and Ae. aegypti (black) mosquitoes (a). Solid lines indicate rearing on sucrose while dotted lines and pill cartoon indicated rearing on antibiotic. The line below shows the time line of the experiments and CFU sampling is indicated by the plates. Infection of CxSm1^RifR and CxSm2^SmR strains into antibiotic-treated or -untreated Ae. aegypti (b). Rifampamicin or spectinomycin was administered to mosquitoes in a sugar meal (capsule indicates antibiotic treatment). For both a and b, the upper panel shows a schematic of experimental design. The line graph indicates the titer of Serratia in mosquitoes, and the pie graph indicates infection prevalence in mosquitoes. For each time point, ten mosquitoes were sampled. Letters indicate significance from the Mann–Whitney test comparing density within a time point. Asterisks indicate a significant difference in Serratia in Cx. quinquefasciatus to Ae. aegypti (a) or antibiotic- and nonantibiotic-treated mosquitoes (b) using a Mann–Whitney test for densinty Fisher’s exact test for prevalence. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Both experiments (a, b) were repeated three times and comparible results were seen between replicates.