Fig. 4: K. stuttgartiensis flux map generated by ¹³C INST-MFA.

K. stuttgartiensis flux map under anaerobic, continuous flow, ammonium and nitrite medium conditions determined by fitting metabolites labelled with ¹³C-formate tracers to a single, statistically acceptable isotopomer network model. Flux values represent the net flux through a given reaction ± standard error defined at 95% confidence. All fluxes are normalized to a net CO₂ uptake rate of q = 100 mmol-C (g DW)⁻¹ h⁻¹ (actual CO₂ uptake rate was 0.186 mmol-C (g DW)⁻¹ h⁻¹). All isotopomer network model reactions are provided in Supplementary Dataset 4. INST-MFA solutions are provided in Supplementary Dataset 5.