Fig. 5: 13C-acetate tracing reveals acetate is oxidized by TCA cycle and not reverse Wood–Ljungdahl pathway. | The ISME Journal

Fig. 5: 13C-acetate tracing reveals acetate is oxidized by TCA cycle and not reverse Wood–Ljungdahl pathway.

From: Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by in vivo 13C and 2H metabolic network mapping

Fig. 5

a Expected dynamic labeling patterns of the TCA cycle based on either Re- or Si-citrate synthase from [2-13C]acetate tracing. Numbers above carbon molecules indicate how many rounds of the TCA cycle have been completed. For simplicity, only new isotopomers produced at each round of the TCA cycle are shown. b 13C-enrichment of selected metabolites during isotope tracer experiments with [2-13C]acetate (red). c Time-series mass isotopomer distributions of selected TCA cycle metabolites during isotope tracer experiments with 13C-acetate. All measured metabolite MIDs represent the average of 2 independent biological replicate experiments. Metabolite MIDs and standard errors can be found in Supplementary Dataset 3. Reaction carbon atom transitions are provided in Supplementary Dataset 4.

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