Fig. 5: Evaluation of benzoate degradation potential in multiple MAGs from the highly enriched BM5, B5, and BL5 cultures. | The ISME Journal

Fig. 5: Evaluation of benzoate degradation potential in multiple MAGs from the highly enriched BM5, B5, and BL5 cultures.

From: Crystalline iron oxides stimulate methanogenic benzoate degradation in marine sediment-derived enrichment cultures

Fig. 5

a Maximum likelihood phylogenomic tree showing closest bacterial relatives of MAGs obtained in this study. b Pathway and genes involved in complete degradation of benzoate to acetate. Genes catalysing the different steps of the benzoate degradation pathways are displayed in different colors for each of the 12 steps in the pathway. c Evaluation of the presence of genes required for complete benzoate degradation and level of completeness of all MAGs retrieved from all enrichment cultures. The color codes for each step in the pathway represent the various genes described in (b). To show presence of a particular gene, the boxes are marked green while the absence of a gene in the obtained MAGs was reflected with red boxes. The intermediate formation of pimelyl-CoA and its subsequent conversion to 3-hydroxypimelyl-CoA was ignored in scheme presented (b) since it was only previously shown for phototrophic α-Proteobacteria Rhodopseudomonas palustris [58]. Eventually R. palustris converts pimelyl-CoA to 3-hydroxypimelyl-CoA like other known benzoate degraders. Quality metrics of the MAGs are available in Tables S2–S5.

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