Table 2 The ten most abundant orthologous proteins identified in single amplified genomes (SAGs) that differ from those in the NSHQ14C Type II metagenome assembled genome (MAG).
Variant protein ortholog annotation (SAGs vs. NSHQ14C MAG) | # of SAGs encoding orthologs with <100% identity to the NSHQ14C MAG | Amino acid identities (SAGs vs. NSHQ14C MAG) | Closest taxonomic affiliation of the most divergent ortholog | Mantel correlation (R) and significance (p) among protein ortholog amino acid dissimilarities and whole genome ANI dissimilarities |
|---|---|---|---|---|
Transposase | 21 | 96.8–98.4% | Methanosarcina mazei | R = −0.01, p = 0.44 |
GDP-mannose 4,6 dehydratase | 17 | 73.9–100% | Methanobacterium congolense | R = 0.07, p = 0.07 |
DDE-type integrase/ transposase/recombinase | 17 | 97.2–100% | Methanobacterium formicicum | R = 0.09, p = 0.21 |
Peptidase C39 | 16 | 76.5–100% | Methanothermus fervidus | R = 0.19, p = 0.02 |
Transposase (IS5 family) | 16 | 96.6–100% | Methanoculleus taiwanensis | R = 0.04, p = 0.23 |
ISNCY family transposase | 15 | 91.6–100% | Methanobacterium subterraneum | R = 0.37, p = 0.01 |
Histidine kinase | 13 | 99.7–100% | Methanobacterium formicicum | R = 0.22, p = 0.07 |
Transposase (IS630 family) | 12 | 97.4–100% | Methanobacterium subterraneum | R = −0.08 p = 0.84 |
MFS transporter | 12 | 99.8–100% | Methanobacterium paludis | R = 0.01 p = 0.43 |
Exosome complex protein Rrp4 | 7 | 99.6–100% | Methanobacterium congolense | R = 0.01 p = 0.50 |
