Fig. 3: Temporal shifts in the community composition of culture DFE with different substrates.
From: Novel dichloromethane-fermenting bacteria in the Peptococcaceae family
Illumina 16S rRNA amplicon sequencing was used to determine DFE community composition (left y-axis) at timepoints across the growth experiments amended with A DCM, B choline, C glycine betaine, and D methanol reported in Figs. 1 and 2. ASVs are reported down to genus level where possible, otherwise taxonomic level is indicated in the legend ([F] = family, [P] = phylum, [C] = class, [O] = order). Reads with <1% abundance were filtered out in QIIME2. Unassigned reads and ASVs consistently <2% relative abundance were classed together as ‘Other’. Substrate concentration (black circles, right y-axis) and a line connecting the mean substrate concentration at each time point is overlaid on the community composition graphs. These are aligned with the time points written on the x-axis, not drawn to scale.
