Fig. 3: Activated smBGCs from eight Streptomyces species under iron-restricted culture conditions. | The ISME Journal

Fig. 3: Activated smBGCs from eight Streptomyces species under iron-restricted culture conditions.

From: Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus

Fig. 3

a Transcriptional landscape of 260 smBGCs in eight Streptomyces species under iron-restricted conditions. The heat maps show log2 expression values of genes in smBGCs. The left side of each heat map represents the position of smBGCs in the sequenced genome. If the median of the normalized expression value of genes in smBGC under iron-restricted conditions was higher than that of actinorhodin BGCs under coculture conditions (341.46), the smBGC was assigned as an expressed smBGC. If the median of log2 fold change value of smBGC-encoded genes was higher than that of actinorhodin BGC under coculture conditions (0.71), the smBGC was assigned as an upregulated smBGC under iron-restricted conditions. Eleven upregulated siderophore BGCs are shown in red, whereas ten upregulated nonsiderophore BGCs are shown in blue. The enlarged heat map shows the log2 expression and log2 fold change values of the 11 upregulated siderophore BGCs and six nonsiderophore BGC-encoded genes, excluding ectoine and melanin BGCs. S. sub S. subrutilus, S. kan S. kanamyceticus, S. ven S. venezuelae, S. ros S. roseosporus, S. rim S. rimosus, S. cin S. cinereoruber, S. co S. coelicolor; S. coer S. coeruleorubidus, CTT pure-cultured in CTT solid medium, IR pure-cultured in iron-restricted CTT solid medium, FC fold change of RNA expression value. b Chemical structures of secondary metabolites produced from the six nonsiderophore BGCs upregulated under iron-restricted conditions. Red-colored chemical residues represent putative interacting sites with metal ions.

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