Fig. 2: H2 oxidation by Hyd-1h. | The ISME Journal

Fig. 2: H2 oxidation by Hyd-1h.

From: The thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV oxidizes subatmospheric H2 with a high-affinity, membrane-associated [NiFe] hydrogenase

Fig. 2

a Total H2-oxidizing activity of the soluble fraction (SF) and the solubilized membrane fraction (SMF) in % of the total activity of the crude extract (CE). Activity was measured spectrophotometrically at 593 nm at 50 °C as reduction of the electron acceptor nitroblue tetrazolium. Error bars indicate standard deviations (n = 3). b High-affinity H2 oxidation by the membrane-associated Hyd-1h with methylene blue as electron acceptor. c H2 oxidation by the purified Hyd-1h with nitroblue tetrazolium as electron acceptor. Hydrogen gas was measured at 40 °C by the membrane-inlet mass spectrometer (MIMS). To determine kinetic parameters, data points were fitted according to Michaelis–Menten kinetics. The red lines show the fitted curves.

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