Fig. 4: Functional analysis of dddD gene, DMS quantification, and ability of E. acroporae to use DMSP as carbon source.

(a) RT-qPCR of the dddD gene from E. acroporae Acr-14T to measure temporal expression change in the presence of 1 mM DMSP (Student’s t test: *p < 0.05, ***p < 0.01). (b) GC-based quantification of DMS released from E. acroporae Acr-14T cultures in the presence of DMSP (1 mM). (c) Growth on 0.1 mM DMSP; E. acroporae strains can use DMSP as its carbon source, whereas E. montiporae CL-33T did not show signs of using DMSP as its carbon source.