Fig. 2: Bacterial diet influences gene expression in P. pacificus.
From: Bacterial vitamin B12 production enhances nematode predatory behavior
a RNA-seq analysis of P. pacificus in response to a diet of Novosphingobium L76 compared with E. coli OP50. The pathways with most significant enrichment (FDR-corrected P < 0−5) in downregulated and (b) upregulated genes are shown. c The dietary sensor Ppa-acs-19.1::RFP is highly expressed in ventral gland, hypodermal and intestinal cells following an E. coli OP50 diet, whereas a N. lin. LE124 diet induces expression only in ventral gland cells. The co-injection marker Ppa-egl-20::RFP is expressed in the tail. d Ppa-stdh-1::RFP is expressed in the intestinal and hypodermal cells with expression strongly upregulated on N. lin. LE124 compared with an E. coli OP50 diet. e Expression of the Ppa-acs-19.1::RFP dietary sensor after feeding on N. lin. LE124 transposon mutants with mutations in vitamin B12 (N. lin. LE124 CbiQ::Tn5), purine (N. lin. LE124 PurH::Tn5), pyrimidine biosynthesis (N. lin. LE124 PryD::Tn5) and nitrogen metabolism (N. lin. LE124 GlnD::Tn5). Mutants increase the expression of the dietary sensor in comparison to a N. lin. LE124 wild-type diet. f Corpse assay of P. pacificus after feeding on various N. lin. LE124 mutants. There is decreased killing efficiency compared to a N. lin. LE124 wild-type diet. N = 10 replicates for each assay.
