Fig. 3: Vitamin B₁₂ containing diet regulates surplus-killing behavior and development.

a Corpse assays showing effects of vitamin B₁₂ supplementation on P. pacificus predation efficiency with P. pacificus fed on either E. coli OP50, N. lin. LE124, N. lin. LE124 CbiQ::Tn5, 500 nM vitamin B₁₂ supplemented E. coli OP50 or 500 nM vitamin B₁₂ supplemented N. lin. LE124 CbiQ::Tn5 prior to assays. b Bite assays showing effects of vitamin B₁₂ supplementation on P. pacificus killing behavior with P. pacificus fed on either E. coli OP50, N. lin. LE124, N. lin. LE124 CbiQ::Tn5, 500 nM vitamin B₁₂ supplemented E. coli OP50 or 500 nM vitamin B₁₂ supplemented N. lin. LE124 CbiQ::Tn5 prior to assays. c Developmental staging of C. elegans and P. pacificus showing percentage of third larval stage (L3), early fourth larval stage (L4), mid fourth larval stage (L4), late fourth larval stage (L4) and young adults on plates after feeding with E. coli OP50, Comamonas DA18877 and N. lin. LE124 for either 45 h (C. elegans) or 56 h (P. pacificus). d Bite assays of P. pacificus fed with E. coli OP50, Comamonas DA18877 and N. lin. LE124. N = 10 replicates for each assay in figure.