Fig. 3: Comparison of RP4 transformability with chlorine-injured bacteria and CaCl2-prepared competent bacteria (n = 3; mean ± SD). | The ISME Journal

Fig. 3: Comparison of RP4 transformability with chlorine-injured bacteria and CaCl2-prepared competent bacteria (n = 3; mean ± SD).

From: Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation

Fig. 3

RP4 (100 ng) was added to 100 µL of 108 cfu/mL chlorine-injured or competent bacteria prepared by the CaCl2 method, according to the heat-shock assay [32, 45]. Bacteria (108 cfu/mL) without exposure to NaClO were selected as controls. *p < 0.01, compared with untreated bacteria; #p < 0.01, compared with CaCl2-prepared competent bacteria.

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