Fig. 1: Conserved far-red specific motifs are present in ApcE2 fragments.

Outlined in red are ApcE2 sequences associated with the far-red cluster, including close homologues found in this study. A phylogeny of the full-length DNA sequences is shown on the left-hand side and in Fig. 5 in more detail. The blue box encompasses conventional (white-light) ApcE sequences. Conventional ApcE is found in chlorophyll f-containing organisms, along far-red ApcE2. The phytochrome-binding cysteine (residue 217, asterisk) is missing in far-red sequences, and instead they show a conserved VIPEDV-like motif (residues 204–209 in C. thermalis). Black boxes and arrows highlight areas covered by the primers designed in this study, both in a far-red-specific area (fw) and a conserved ApcE-specific area (rev).