Table 1 Main sets of primers used in this study.

From: Global distribution of a chlorophyll f cyanobacterial marker

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  1. af forward, r reverse. M refers to medium degeneracy in comparison with other primers tested in this study (Table S2). t represents tags. Asterisks mark versions of the same primer with an alternate tag.
  2. bAll degenerate primers had 5′ tags. Red boxes mark any base (N); blue boxes mark bases with a degeneracy of 3 (H = not G; D = not C; V = not T); green boxes mark bases with a low degeneracy (Y = C/T; R = A/G).
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