Fig. 2: Bulk tissue assimilation of the four food sources and contribution of host and symbiont cells to total uptake.

Rates of carbon (C) and nitrogen (N) assimilation of the four food sources a glucose, b amino acids, c algal DOM, and d bacteria into the bulk tissue of the HMA sponge A. aerophoba and LMA sponge D. avara. Rates presented as µmol C or N assimilated per mmol C or N sponge tissue per hour. Significant differences in C or N assimilation between the two sponge species are marked (*) for each food source (significance level P(perm) < 0.05). Boxplots depict the 25th percentile, median, and 75th percentile overlaid with the raw data points (n = 20). (e–l) Percent contribution of host sponge cells (dark colors) and symbiont microbes (light colors) to total assimilation of the four food sources for both carbon (green) and nitrogen (orange) as determined by isotopic enrichment of separated cell fractions. .