Fig. 3: NanoSIMS visualization of the relative enrichment in 13C or 15N in sponge host and symbiont cells. | The ISME Journal

Fig. 3: NanoSIMS visualization of the relative enrichment in 13C or 15N in sponge host and symbiont cells.

From: Heterotrophy in the earliest gut: a single-cell view of heterotrophic carbon and nitrogen assimilation in sponge-microbe symbioses

Fig. 3

The measured ratios (i.e., 13C12C−/12C12C− and 15N12C−/14N12C−, respectively) are shown with a rainbow color-scale (hue saturation intensity) ranging from blue (natural abundance) to pink (most enriched regions) in A. aerophoba (HMA) and D. avara (LMA) tissue after the 3-h isotopic pulse with the four different 13C and 15N-labeled food sources: a, b glucose, c, d amino acids, e, f algal DOM, and g, h bacteria. Note the different y-axis scale for the bacterial food source (g, h). DOM dissolved organic matter, POM particulate organic matter, c host choanocyte cell, hs hotspot of enrichment in host choanocyte cell, m host mesophyll cell, b symbiont bacteria. Scale bars are 5 µm.

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