Fig. 4: Single-cell quantification of above-background 13C and 15N enrichment in the HMA sponge A. aerophoba and LMA sponge D. avara. | The ISME Journal

Fig. 4: Single-cell quantification of above-background 13C and 15N enrichment in the HMA sponge A. aerophoba and LMA sponge D. avara.

From: Heterotrophy in the earliest gut: a single-cell view of heterotrophic carbon and nitrogen assimilation in sponge-microbe symbioses

Fig. 4

Four regions of interest (ROIs) were analyzed by NanoSIMS: (1) symbiont bacteria, (2) hotspots of enrichment in host choanocyte cells, (3) host choanocyte cells, and (4) host mesohyl cells. Above-background enrichment (‰) of δ13C (a–d) and δ15N (e–g) for each ROI type after the 3-h isotopic pulse with the four labeled food sources. A minimum of n = 20 ROIs were analyzed for each ROI type. Note the different log10 y-axis scales. Gray horizontal lines depict background enrichment of control samples. ‡ denotes significant difference in individual ROI types between the HMA and LMA sponge (P(perm) < 0.05). Pie charts depict the proportion of ROIs enriched in 13C or 15N for each of the three cell types of interest: B symbiont bacteria, C choanocyte cells, M mesohyl cells.

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