Fig. 5: Comparison of metagenome-assembled genomes in Lake Tanganyika and Lake Baikal.

A Histogram of the ANI values when doing pairwise comparison of full-genome MAGs from Lake Tanganyika vs. Lake Baikal. The vertical dashed lines correspond to the mean value (Baikal vs. Baikal: 79.98%, Tanganyika vs. Baikal: 75.63%, and Tanganyika vs. Tanganyika 76.60%). B Zoom in ANI values above 80% only. There are slightly more ANI above the 97% ANI for Baikal vs. Baikal, than Tanganyika vs. Tanganyika. Two genomes from Baikal had 100% ANI, two genomes from Tanganyika (K_Offshore_surface_m2_005 and K_DeepCast_100m_m2_150) shared 99.91% ANI. The highest % ANI between a MAG from Tanganyika vs. Baikal was among M_surface_10_m2_136 and GCA_009694405.1_ASM969440v1_genomic (90.19% ANI), which were Candidatus Nanopelagicaceae bacterium, an Actinobacteria. C Of the pairs in “Tanganyika vs. Baikal” category, showing the number of pairwise comparisons for each % ANI value, organized by taxonomic group. In C, all the x-axes are “% ANI.” For example, there is only one pair of Bacteroidetes, Cyanobacteria, and Euryarchaeota from the two lakes that have >75% ANI.