Fig. 4: C. albicans–C. freundii growth interactions are altered by priority effects.
From: Priority effects dictate community structure and alter virulence of fungal-bacterial biofilms
A Relative abundance plots (full scale and zoomed) of in vitro C. albicans–C. freundii biofilms growth in RPMI-1640 media at 37 °C using both staggered and simultaneous inoculation models. Stacked bars calculated from means of CFU data shown in (C–E). B Summary of priority effects on growth in co-culture subtracted from time-matched mono-culture controls. Data points show mean differences with 95% confidence intervals calculated from CFU data shown in (C–E) for each microbe using a one-way ANOVA followed by Tukey’s HSD test. Differences are significant if confidence intervals do not include 0. C CFUs for C. albicans–C. freundii biofilms inoculated simultaneously (no priority effect) and grown for 48 h and time-matched mono-culture controls (48 h). D CFUs for C. albicans–C. freundii biofilms where C. albicans was inoculated 24 h before C. freundii (C. albicans exerts priority effect) and grown for 48 h, and time-matched mono-culture controls (C. albicans 48 h, C. freundii 24 h). E CFUs for C. albicans–C. freundii biofilms where C. freundii was inoculated 24 h before C. albicans (C. freundii exerts priority effect) and grown for 48 h, and time-matched mono-culture controls (C. freundii 48 h, C. albicans 24 h). For panels (C–E), each data point represents one replicate well; horizontal bars show means of ≥9 replicates; data are pooled from n ≥ 3 independent experiments. * p < 0.05, ** p < 0.0001, ns: not significantly different.
