Fig. 5: C. freundii and S. aureus compete when attaching to established C. albicans biofilms.
From: Priority effects dictate community structure and alter virulence of fungal-bacterial biofilms
A Relative abundance plots (full scale and zoomed) of in vitro biofilms in RPMI-1640 media at 37 °C where bacteria (C. freundii, S. aureus, or C. freundii + S. aureus) are inoculated onto pre-established 48 h C. albicans biofilms and are allowed to grow for an additional 24 h. Stacked bars calculated from means of CFU data shown in (C). B Summary of interbacterial competition effects on growth on pre-established C. albicans biofilms subtracted from mono-colonizer controls. Data points show mean differences with 95% confidence intervals calculated from CFU data shown in (C) for each microbe using a one-way ANOVA followed by Tukey’s HSD test. Differences are significant if confidence intervals do not include 0. C CFUs for fungal-bacterial biofilms where bacteria (C. freundii, S. aureus, or C. freundii + S. aureus) are inoculated onto pre-established C. albicans 48 h biofilms. For panel (C), each data point represents one replicate well; horizontal bars show means of ≥9 replicates; data are pooled from n ≥ 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. D Correlation plot between CFU counts from ex vivo and in vitro models. Data points are colored by organism for each condition and represent means with error bars showing standard deviation. Dashed line represents line where y = x. # indicates S. aureus counts in tri-culture with C. albicans and C. freundii.
