Fig. 4: Hierarchical clustering of target strains by O-specific antigen biosynthetic cluster similarity, overlaid with tailocin sensitivity data.
From: Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins
In all, 130 Pseudomonas strains were clustered by the similarity of their OSA clusters, assessed as Jaccard distance of orthogroups (OrthoFinder v2.2.7 [71]) found in the OSA cluster genes. See Methods for details on our approach to delineating OSA clusters. Tailocin-producing strain labels are highlighted in color. Shaded boxes at the outer edge of the tree indicate sensitivity of that strain to the correspondingly colored tailocin. To aid discussion of three clades of strains with interesting features, we named those clades after the most closely related species by 16S rRNA similarity: E clade (P. extremorientalis); TKP clade (P. sp. TKP); SM clade (P. silesiensis/P.mandelii). In this clustering, TKP strains are separated into two groups: TKPS (Pse11 Tailocins sensitive) and TKPR (resistant). For a phylogenetic tree of these strains, see Supplementary Fig. S8. For a comparative illustration of the OSA clusters encoded by these strains, see Supplementary Fig. S9 (for a full list of orthogroups, see Supplementary Table S22). For the killing matrix in table format, see Supplementary Table S6.
