Fig. 3: NanoSIMS analysis of cells from microcosms amended with 13C-enriched alginate. | The ISME Journal

Fig. 3: NanoSIMS analysis of cells from microcosms amended with 13C-enriched alginate.

From: Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria

Fig. 3

Cells were hybridized with CF319a probe targeting Flavobacteriales (A–F) or GAM42a probe targeting Gammaproteobacteria (G–L), using fluorine-containing tyramides. Rows show parallel acquisitions of the same region. Columns display secondary ion images of 32S as total biomass indicator (A, D, G, and J; a.u: arbitrary intensity unit), the ratio 19F/32S as a marker for cell identity (B, E, H, and K) and the 13C atom fraction inferred from secondary ions (13C14N, 12C14N) as indicator of 13C incorporation from alginate (C, F, I, and L), in HSI (Hue-Saturation-Intensity) color scale. Scale bars: 2 µm.

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