Fig. 1: Acidithiobacillia class genus- and species-level taxons defined on the basis of whole-genome relatedness indexes based on amino acid and nucleotidic sequence identity pairwise comparisons. | The ISME Journal

Fig. 1: Acidithiobacillia class genus- and species-level taxons defined on the basis of whole-genome relatedness indexes based on amino acid and nucleotidic sequence identity pairwise comparisons.

From: Genomic evolution of the class Acidithiobacillia: deep-branching Proteobacteria living in extreme acidic conditions

Fig. 1

A Amino acid identity values calculated with CompareM (AAICM; Table S3a; https://github.com/dparks1134/CompareM]) vs 16S rRNA gene sequence pairwise identities (Table S3b) showing acknowledged genus (% AAI > 70%) and species (%16 S rRNA gene > 98.7%) cutoff values [63]. B Histogram of AAI% values colored by the taxonomic rank of each pairwise comparison. C Average nucleotide identity (ANI) heatmap using ANIb and ANIm alignment algorithms threshold [47, 63]. D Digital DNA–DNA hybridization index (dDDH) box plot showing divergence between strains assigned to known and novel genomic species (Table S4) with respect to the species cutoff [48, 49], [red line: % dDDH > 70%]. The acronyms presented on the right have been subsequently used throughout the manuscript.

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