Fig. 4: Phylogenetic placement of 15 MAGs recovered from soils.

Tree clades containing 13C-labeled MAGs detected in this study are shown in red. Known biphenyl-degrading species are highlighted in green. The genomic tree was built from the concatenation of 120 bacterial marker genes. Filled circles on tree nodes indicated bootstrap values >70%. Scale bar, amino acid substitutions per site. The distribution of bph genes is shown in the right panel. Filled and empty circles represent the presence or absence of bph genes. The gene distribution is not shown for lineages without detectable bph homologs. Abbreviations: bphA: biphenyl dioxygenase subunit alpha; bphE: biphenyl dioxygenase subunit beta; bphF: biphenyl dioxygenase ferredoxin component; bphG: biphenyl dioxygenase system ferredoxin/NAD+ reductase component; bphB: cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase; bphC: 2,3-dihydroxybiphenyl 1,2-dioxygenase; bphD: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; bphI: 4-hydroxy-2-oxovalerate aldolase; bphH: 2-oxopent-4-enoate hydratase; bphJ: acetaldehyde dehydrogenase.