Fig. 1: Growth and activity of methanogens on different iron and sulfur sources.
From: Reductive dissolution of pyrite by methanogenic archaea
Production of biomass, methane, and sulfide in cultures of Methanococcus voltae provided with formate (a, c, e, respectively) or Methanosarcina barkeri provided with methanol and acetate (c, d, f, respectively) as the methanogenesis substrate when grown with different iron and sulfur sources in defined medium. Legends in a and c and in b and d are the same. Ferrous iron and sulfide or cysteine were added to final concentrations of 26 µM and 2 mM, respectively. Laboratory synthesized pyrite was added to a final concentration of 2 mM Fe. CH4, total methane; HS−, total sulfide; Fe(II), ferrous iron; Cys, cysteine; FeS2, pyrite. Data shown are the mean values for each condition with error bars reflecting the standard deviation of three replicate biological reactors; a single reactor for abiotic controls was monitored and thus error bars are not presented. Total HS− was monitored only in the FeS2 growth conditions. See Extended Data File 1 for CH4 and HS− data.
