Fig. 5: The distribution of carbon assimilating groups within the rock, sediment, and seawater samples.

The heatmap shows the relative abundance of 16S rRNA gene sequences per group per sample (purple = more, light blue = less). Within the qSIP incubations (right-hand side), groups that had OTUs with statistically significant 13C-assimilation in qSIP are surrounded by a yellow box. The histograms on the right side of the plot show the average 13C-EAF across all qSIP incubations per group. An asterisk indicates that this group had a significant 13C-EAF within a qSIP incubation with added H2 (no asterisk means labeling occurred only in qSIP incubations without H2). The histograms at the top of the heatmap show the average 13C-EAF across all OTUs within each qSIP incubation, error bars represent standard deviations. The analysis shows that the majority of 13C-assimilating groups were rock-associated and that more rock-associated groups became labeled in the presence of H2 (middle of the heatmap), compared to the seawater-associated groups (at the bottom of the heatmap).