Fig. 7: Distribution of HypE and nirS in metagenomes.

Phylogenetic analyses (RAxML) of all detected HypE (A) and nirS (B) ORFs based on alignment lengths of 442 and 638 amino acids, respectively. Note that the separation of seawater (pink highlighted) and rock-associated (light blue highlighted) clades. Panels (C) and (D) show the relative abundance (% reads mapping, length normalized) within the different metagenomes to ORFs with best BLASTp similarity to the NiFe-hydrogenase assembly locus HypABCDEF (C) and nirS (D), respectively. Circles on nodes represent bootstrap values (black > 90%, gray > 70%, white > 50%).