Fig. 2: Overlap of substrate depletion profiles between individual OMM¹² strains.

(A) Depletion profiles of substrates after bacterial growth to stationary phase in AF medium were determined by untargeted MS from three independent experiments. All metabolomic features (rows) that significantly decreased (p < 0.05 compared to fresh media) compared to fresh medium for at least one of the twelve strains are shown in red. Dark-red indicates strong depletion, while white indicates no depletion of the metabolomics feature. Hierarchical clustering of strain-specific profiles as well as metabolomic features reveal profile similarities between phylogenetically similar strains. (B) Bar plot showing the total number of significantly (p < 0.05 compared to fresh media) depleted metabolomic features in AF medium for the individual strains. (C) Pairwise overlap in depleted metabolomic features relative to the total number of depleted metabolomic features (shown in B) of every individual strain. E.g., E. faecalis KB1 shares 33 metabolomic features from its set of 370 depleted metabolomic features with B. animalis YL2, corresponding to 8.9%. As B. animalis YL2 in contrast only depletes 128 metabolomic features in total from AF medium, this corresponds to an overlap of 25.8% of shared metabolites between B. animalis YL2 and E. faecalis KB1 relative to the total set of B. animalis YL2 depleted metabolomics features. (D) Euler diagram depicting number of depleted metabolomic features and overlap within the full consortium as grouped by bacterial phyla. Size of the ellipses denotes the number of depleted features, size of overlap between ellipses denotes number of features that are shared when comparing all individual profiles. Where several ellipses overlap, depleted metabolomic features are shared by more than two phyla. Colors indicated in the legend denote areas of metabolomics features that are unique to a phylum (indicated in percent of total depleted metabolomics features), overlapping areas are indicated in muted colors.