Fig. 3: Characterization of the sorghum root-associated microbiome after the high-throughput phenotyping assay. | The ISME Journal

Fig. 3: Characterization of the sorghum root-associated microbiome after the high-throughput phenotyping assay.

From: Identification of beneficial and detrimental bacteria impacting sorghum responses to drought using multi-scale and multi-system microbiome comparisons

Fig. 3

a The clustering of microbiome samples using unsupervised UMAP, with colors and shapes showing the drought and microbial treatments, respectively. b Phylum-level distribution of the microbial microbiota across treatments. c The OTU abundance of Variovorax and Arthrobacter OTUs at the conclusion of the assay under drought. The dots represent the OTU abundance in different samples with colors showing the microbial treatments. The horizontal bars within boxes represent medians. The tops and bottoms of the boxes represent the 75th and 25th percentiles, respectively. The upper and lower whiskers extend to data no more than 1.5× the interquartile range from the upper edge and lower edge of the box, respectively. Pairwise t-tests were performed between SynCom treatments. The p values for select comparisons are shown and all others were not significant at the alpha of 0.05. The numbers of replicated samples n ≥ 8. d The numbers of OTUs associated to both plant phenotypes. Colors represent the OTU groups with same association directions. e Phylum-level distribution of the plant phenotype-associated microbiota within drought treatments. f, g The change-point model fitting between OTU abundance and plant phenotypes (f plant size; g shoot fresh weight) for OTU194097 Arthrobacter strain. Gray dots indicate samples that did not meet the abundance threshold.

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