Fig. 4: Metagenomic functional diversity in skeletal compartments of G. edwardsi and P. lutea.

Ordination plots based on the composition of KEGG orthologs (KOs) involved in A overall metabolism, B carbon metabolism, and C nitrogen metabolism. D Shannon diversity of metabolism KOs. Significant differences between species and skeletal compartments are denoted by letters above the boxes. E Volcano plots depicting differentially abundant KEGG orthologs (KO) involved in metabolism between G. edwardsi and P. lutea. Each dot represents a KO and differentially abundant KOs are colored by metabolic processes. KOs that are not differentially abundant between species are colored in black. Dashed red lines represent thresholds for FDR-adjusted p value < 0.05 and effect sizes (1 and −1) represented by the Beta statistic obtained from ANCOM-BC. F Differentially abundant carbon metabolism KEGG L3 pathways between G. edwardsi and P. lutea. Clr-transformed pathway counts were plotted and z-normalized by KEGG L3 pathways (rows). Left bars indicate the effect size represented by the W ANCOM-BC statistic and colors represent the coral species in which the process was enriched. Colored boxes represent the affiliation to KEGG L2 pathways.