Fig. 1: Schematic overview for collecting output libraires for the Tn-seq analysis of root colonization arsenal of Sinorhizobium fredii on legume and cereal plants.
From: Rhizobial migration toward roots mediated by FadL-ExoFQP modulation of extracellular long-chain AHLs
Three independent mariner transposon insertion libraries of S. fredii CCBAU25509 (SF2) were used as input inoculants in three independent experiments. Their 1h-post-inoculation (F1h) and 7d-post-inoculation (F7d) samples on the filter of plant culture dish were used as control samples to those rhizoplane samples of four test plant species (CS7d, WS7d, R7d, and Z7d). To facilitate Tn-seq library construction, all output samples were subject to cultivation in the TY rich medium for 32 h, and the input libraries were cultivated under the same condition as control (TY).
