Fig. 7: Genotype-dependent variation in extracellular and intracellular AHLs.
From: Rhizobial migration toward roots mediated by FadL-ExoFQP modulation of extracellular long-chain AHLs
A Detection of extracellular AHLs from rhizobia (central colony) using biosensor A. tumefaciens KYC55(pJZ372)(pJZ384)(pJZ410) (circle) on the TY plate (0.5% agar, Congo red, and X-gal). B β-galactosidase activity of A. tumefaciens KYC55(pJZ372)(pJZ384)(pJZ410) cells collected from (A). C Intra- and extracellular AHL concentrations determined using the HPLC-Q-Exactive-PRM-MS approach. NF, not found. D A working model for the transport of long- and short-chain AHLs through FadL and ExoFPQ integrated in membranes. A predicted repression of long-chain AHL biosynthesis by short-chain AHLs is shown. Significant difference between means is indicated in (B) (*, p < 0.05; ***, p < 0.001; t-test), and (C) (different letters in brackets; ANOVA followed by Duncan’s test, alpha = 0.05) based on three independent experiments. Error bars represent SEM (B) or SD (C).
