Fig. 8: Exogenous long-chain AHLs enhance rhizobial migration ability toward roots.
From: Rhizobial migration toward roots mediated by FadL-ExoFQP modulation of extracellular long-chain AHLs
A Intra- and extracellular AHL concentrations determined using the HPLC-Q-Exactive-PRM-MS approach. NF not found. Different letters indicate significant differences between means (±SD, three independent experiments) based on ANOVA followed by Duncan’s test, alpha = 0.05. B Intracellular short-chain AHLs of traI sinI and traI sinI exoF mutants in the presence of 100 ng/ml exogenous 3-OXO-C8-HSL. C Intracellular long-chain AHLs of traI sinI and traI sinI fadL mutants in the presence of 100 ng/ml exogenous 3-OXO-C14-HSL. D–F Exogenous long-chain AHLs enhance rhizobial migration ability toward roots (2 μl long-chain AHL solution, containing 45 ng 3-OXO-C12-HSL, 52 ng C14-HSL, and 1 μg 3-OXO-C14-HSL, according to the extracellular AHL composition detected from the fadL mutant in Fig. 7C). D A schematic overview of experimental design showing inoculation of rhizobia (WT/mutants) and AHLs (or 0.8% NaCl as the negative control) at symmetric distances to root. E, F Long-chain AHLs enhance rhizoplane colonization by WT (E), the traI sinI and exoF mutants (F). Significant difference between means (±SEM, three independent experiments) are indicated in (B–F) (*, p < 0.05; **, p < 0.01; ***, p < 0.001; t-test).
