Fig. 3: Gene expression for central carbon and energy metabolisms in M. tundripaludum SV96^T.

Heatmaps indicate changes in the relative abundance of transcripts in the transcriptomes at 8, 15, 21, and 27 °C. The color scale reflects z-score normalized relative abundances, with black being lowest relative abundance, followed by gray, yellow, orange, and red, corresponding to increasing relative abundances. Significant differences (p < 0.05) in transcript relative abundances are displayed in square plots to the right of each heatmap, purple indicating significant difference between 15 °C, and 8, 21 or 27 °C, respectively, when moving from left to right. Orange indicates significant differences between 8 °C and 21 and 27 °C. Dark blue indicates significant differences between 21 and 27 °C. All p values were estimated with a Wald test, implemented in DESeq2, and corrected for multiple testing using Benjamini Hochberg correction which adjusts for the false discovery rate. Purple arrow in metabolic pathways indicates upregulation at 15 °C, meaning that at least one of the genes encoding the enzyme responsible for catalyzing the reaction represented by this pathway step is significantly higher expressed at 15 °C than at two or three of the other temperatures. Numbers next to heatmaps refer to corresponding genes in SD C: Table C1 and corresponding metabolic step illustrated in the figure. All adjusted p values, normalized counts, genome IDs (a unique identifier that allows tracking of each gene to the genome annotation available in https://mage.genoscope.cns.fr/microscope), full protein names and E.C. numbers for the genes are provided in SD C: Table C1.