Fig. 5: Glycogen synthesis and storage in M. tundripaludum SV96^T.

Gene expression for glycogen synthesis (A), and glycogen accumulation per cell (B) in M. tundripaludum SV96^T. Heatmaps (A) indicate changes in the relative abundance of transcripts in the transcriptomes at 8, 15, 21, and 27 °C. The color scale reflects z-score normalized relative abundances, with black being lowest relative abundance, followed by gray, yellow, orange, and red, corresponding to increasing relative abundances. Significant differences (p < 0.05) in transcript relative abundances are displayed in square plots to the right of each heatmap, purple indicating significant difference between 15 °C and 8, 21 and 27 °C, respectively, when moving from left to right. Orange indicates significant differences between 8 °C and 21 and 27 °C. Dark blue indicates significant differences between 21 and 27 °C. All p values are estimated with a Wald test, implemented in DESeq2, and corrected for multiple testing using Benjamini Hochberg correction which adjusts for the false discovery rate. Blue arrows indicate that at least one of the genes encoding the enzyme responsible for catalyzing the reaction represented by this pathway step is significantly higher expressed at 8 °C than at two or three of the other temperatures. Red arrow indicates significantly higher expression at 27 °C. All adjusted p values, normalized counts, genome IDs (a unique identifier that allows tracking of each gene to the genome annotation available in https://mage.genoscope.cns.fr/microscope) and full protein names are given in SD C: Table C6. Bar graph (B) show the µg glycogen per cell in exponential state culture, estimated from a cell lysate with a known cell number. Considering the possibility of incomplete lysis, the numbers are likely conservative estimates. Error bars indicate standard deviation. Numbers above bars indicate to which of the other bars there are significant differences (p < 0.05).