Fig. 2: The enrichment of incorporators of added 13C-biomass in heavy rRNA fractions and the temporal labeling patterns. | The ISME Journal

Fig. 2: The enrichment of incorporators of added 13C-biomass in heavy rRNA fractions and the temporal labeling patterns.

From: Active predation, phylogenetic diversity, and global prevalence of myxobacteria in wastewater treatment plants

Fig. 2

13C-labeled prokaryotic (A) and micro-eukaryotic (B) genus-level taxa were identified by SIP in the microcosms added with E. coli and P. putida after one, two, and four days of incubation. Enrichment factor (EF) was calculated for microorganisms using heavy and light rRNA gradient fractions of the 13C- and 12C-microcosms to infer 13C-labeling. Taxa with an EF > 0.1 in at least one of the treatment groups at one sampling time point was considered labeled. The area of circles indicates the relative sequence abundance of the labeled taxa in heavy 13C-rRNA. The negative EFs and positive EFs < 10−1.5 were arbitrarily set to 10−1.5 for a neat graphical display. Heatmap of the 13C-incorporating prokaryotic (C) and micro-eukaryotic ASVs (D) with relative sequence abundance >1% in the heavy rRNA fractions of at least one of the 13C-E. coli and 13C-P. putida microcosms at a sampling point.

Back to article page