Fig. 3: In situ relative abundance of Myxococcota and Bdellovibrionota in aerobic and anaerobic sludge at a local WWTP (WWTP01) based on sampling over two years.

The abundance of the abundant genera belonging to Myxococcota and Bdellovibrionota in aerobic and anaerobic sludge were compared according to amplicon sequencing-based analysis of bacterial 16S rRNA gene V3-V4 region. The top 10 abundant genus-level taxa across samples collected from eight samplings are shown, with the putative predators identified by SIP in the microcosm experiment highlighted. The asterisk denotes significant difference in relative abundance between aerobic and anaerobic sludges (p < 0.05, paired samples Wilcoxon test, n = 8).