Fig. 2: Genome properties via CheckM of the major phylogenetic delineations (also corresponding to gene cluster content) in Nitrospirota and Nitrospinota show that basal clades (purple) have smaller genome length and higher coding density than later brancing clades (green).

Estimated genome length y-axis scale is 106 base pairs, and coding density y-axis is as percent. Red asterics denote results that show significant differences between metrics in the clades by a ANOVA (p value < 0.05). For Nitrospirota the p values for genome length and coding density are 3.63e−20 and 3.25e−20, respectively. For Nitrospinota the p values for genome length and coding denisty are 0.13 and 0.0011, respectively.