Fig. 6: Metabolism of strain YIM 72310^T as determined from physiological tests, isotopically labeled ¹³CO₂ production and assimilation assays, exometabolomics and genomic predictions.

a Growth stimulation tests performed through addition of carbon sources to 2R2AW at a concentration of 0.05% w/v, after 8 days of growth as measured by optical density at 600 nm. Statistically significant stimulation (indicated with an asterisk) was determined based on unpaired t-tests (p < 0.05) compared to controls for the respective experiments. b Average relative ¹³CO₂ production from growth on ¹³C-labeled substrates (triplicate incubations ± standard deviations). Statistical significance (indicated with asterisks) was determined through comparison to the negative control, with no ¹³C-labeled substrates, with Dunn tests. c Assimilation of ¹³C-labeled substrates was calculated from nanoSIMS analyses of individual cells (see Supplementary Note S7). Substrate biomass assimilation of individual cells, shown in each data point was calculated using C_net and plotted with a boxplot overlay for the average percent incorporation and the upper and lower quartile ranges. Significant assimilation of ¹³C (indicated with asterisks) was determined through comparison to the negative control with Dunn tests. d Dot matrix summarizing the metabolic capabilities of YIM 72310^T. Substrates and products identified through exometabolomics are indicated with yellow dots, while supporting evidence based on ¹³CO₂ production (blue dots) and assimilation (pink dots) experiments, genomic predictions (purple dots) and growth tests (green, orange, and gray dots) are also indicated. P1 Pyruvate, first carbon atom is labeled with ¹³C; P2,3 Pyruvate, second and third carbon atoms are labeled with ¹³C, FAs fatty acids, IAA indole-3-acetic acid, PAA phenylacetic acid, AAs amino acids, Nts nucleotides, Nt nucleotide.