Fig. 5: Glutathione-S-transferases (GSTs) of B. bassiana and their gene inducibility and enzymatic activities with three ITCs.

A Phylogenetic analysis of the seventeen B. bassiana GSTs shows division into twelve groups, eight of which represent existing subfamilies. The BbGST amino acid sequences were aligned and a UPGMA tree was generated. The branch labels represent the expected amino acid substitutions per site. B Heatmap showing the expression of GST-encoding genes in B. bassiana (relative to Bbactin, n = 5). The data show log₂ fold-change of GST gene expression level in B. bassiana incubated with 4MSOB-ITC (1a), A-ITC (2a), and 2PE-ITC (3a), relative to the expression in the B. bassiana control group. Only statistically significant differences are indicated. C Specific activity ±SE (µmol substrate consumed · mg⁻¹ enzyme · min⁻¹) of B. bassiana GSTs was determined for 4MSOB-ITC (1a), A-ITC (2a), and 2PE-ITC (3a) (n = 4). Specific activity values corrected for non-enzymatic conjugation are listed in Table S5. D PCA plot showing the variation of GST activities with different ITCs. Vectors indicate the direction and strength of each GST protein activity relative to the overall distribution. Statistically significant differences between means (±SE) were determined by two-tailed t tests (p ≤0.05) in B, and by Tukey HSD tests in conjunction with two-way ANOVA in C, and are denoted by asterisks and lowercase letters.