Table 1 Kinetic constants of B. bassiana GST enzymes with the substrates 4MSOB-ITC (1a), A-ITC (2a) and 2PE-ITC (3a).

Enzymes	Substrates	V_max	K_M	k_cat	k_cat/K_M
Enzymes	Substrates	µmol · mg^-1 · min^-1	µM	s^-1	mM^-1 · s^-1
BbGSTUre2p2	4MSOB-ITC (1a)	1.09 ± 0.54	1210.99 ± 889.50	0.45 ± 0.23	0.37 ± 0.19
	A-ITC (2a)	0.44 ± 0.12	487.23 ± 266.11	0.18 ± 0.05	0.37 ± 0.10
	2PE-ITC (3a)	0.15 ± 0.02	223.06 ± 86.81	0.06 ± 0.01	0.28 ± 0.05
BbGSTUre2p3	4MSOB-ITC (1a)	1.04 ± 0.27	747.36 ± 338.25	0.47 ± 0.12	0.63 ± 0.17
	A-ITC (2a)	0.59 ± 0.24	831.61 ± 572.57	0.27 ± 0.11	0.32 ± 0.13
	2PE-ITC (3a)	0.08 ± 0.01	71.76 ± 38.91	0.04 ± 0.01	0.49 ± 0.08
BbGSTU	4MSOB-ITC (1a)	0.40 ± 0.09	365.55 ± 184.07	0.24 ± 0.05	0.67 ± 0.15
	A-ITC (2a)	2.64 ± 1.33	1178.31 ± 884.78	1.59 ± 0.80	1.35 ± 0.68
	2PE-ITC (3a)	0.44 ± 0.09	391.89 ± 159.63	0.27 ± 0.06	0.68 ± 0.14
BbGSTe1	4MSOB-ITC (1a)	3.86 ± 1.80	1220.41 ± 839.36	1.77 ± 0.82	1.45 ± 0.68
	A-ITC (2a)	0.41 ± 0.05	312.75 ± 100.06	0.19 ± 0.02	0.59 ± 0.08
	2PE-ITC (3a)	1.90 ± 0.76	860.76 ± 561.56	0.88 ± 0.35	1.02 ± 0.41
BbGSTN21	4MSOB-ITC (1a)	3.96 ± 1.34	1132.00 ± 578.29	1.66 ± 0.56	1.47 ± 0.50
	A-ITC (2a)	2.39 ± 0.58	448.48 ± 226.89	1.00 ± 0.24	2.23 ± 0.54
	2PE-ITC (3a)	0.97 ± 0.86	1191.89 ± 1464.92	0.41 ± 0.36	0.34 ± 0.30
BbGSTN3	4MSOB-ITC (1a)	1.06 ± 0.30	884.72 ± 412.37	0.72 ± 0.21	0.82 ± 0.23
	A-ITC (2a)	1.61 ± 0.78	784.38 ± 647.72	1.09 ± 0.53	1.39 ± 0.68
	2PE-ITC (3a)	0.32 ± 0.11	396.52 ± 270.03	0.22 ± 0.08	0.55 ± 0.19

Michaelis-Menten constants (±SE) were determined by nonlinear regression of enzymatic activities measured using variable ITC concentrations (25 µM to 800 µM) at saturating GSH concentration (4 mM), n = 4.

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