Fig. 4: Some predation-resistant Pseudomonas strains protect edible bacteria from predation.

A 14P_8.1_Bac3-GFP, B 7P_10.2_Bac1-GFP, and C K. pneumoniae-GFP CFUs recovered after 7 d co-culture with D. discoideum AX4. Edible bacteria were grown on SM/5 agar with a predation-resistant Pseudomonas strain (20P_3.2_Bac4, 6D_7.1_Bac1, or 18P_8.2_Bac1) and D. discoideum or with buffer and D. discoideum (control). Edible bacteria CFU were quantified by collecting cells from plates and spotting serial dilutions on selective media. Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test with individual variances comparing each sample to the control. *p ≤ 0.05.