Fig. 5: In planta SIP production from E. aphidicola strain.

A 12-day-old wheat seedling with the first leaf labelled FL. B CFU and PFU of two treatments inoculated on the first leaf of 12-day-old Heerup seedlings. The first treatment (B01_5) is E. aphidicola strain B01_5 washed twice in PBS buffer, while the second treatment (control) is the cell-free supernatant of washed B01_5. Leaves were washed in SM buffer, and colonies counted on Pseudomonas selection agar, while plaques were counted using soft-agar overlays with susceptible strain B01_10 as host. Each data point is the mean of four biological replicates, with error bars representing max/min values. The detection limit for the mean of four biological replicates is 1.5 PFU or CFU / leaf (denoted by black dotted line), with datapoints below this artificially set at 1.3 for clarity. C The ratio PFU/CFU for the B01_5 cell treatment, along with the recorded in vitro PFU/CFU ratio denoted by the blue dotted line. Orange line connects the mean of each data point, while all data points are shown as black dots.