Fig. 2

Overview of PCR products of designed primers. Locations of 5′-end and 3′-end primer sequences for HLA-A, HLA-B, and HLA-C genes are shown in hg19 coordinated with the target and co-amplified PCR products. Primer names “A–F” and “A–R” stand respectively for forward and reverse primers for HLA-A gene. For each of “A–F(1)”, “C–F(1)”, and “B–R(1)”, the edit distance of the primer sequence from the corresponding sequence in hg19 reference is shown in parenthesis after the primer name