Fig. 2: Contribution of Wnt/β-catenin responsive TBE sites to Nlgn3 promoter activity. | Translational Psychiatry

Fig. 2: Contribution of Wnt/β-catenin responsive TBE sites to Nlgn3 promoter activity.

From: Wnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 gene

Fig. 2

a Genomic context of mouse Nlgn3 promoter and different Nlgn3 chimeric promoter constructs (pNL3Mm). Circles indicate potential TBE consensus sequences in the predicted regulatory sequence of Nlgn3 gene. Distances are in reference to the transcription start site (TSS). b Transient transfection of increasing doses (30, 60, and 90 ng) of Nlgn3 chimeric promoter constructs pNL3Mm2.8, pNL3Mm1.4, and pNL3Mm0.3 in HEK293 cells for 24 h. c Transient co-transfection of pNL3Mm2.8 kb or pNL3Mm1.4 with increasing doses of constitutively active β-catenin (S33Y) for 24 h. d Effects of dominant negative delta-TCF4 (ΔTCF4) on β-catenin induced pNL3Mm1.4 promoter activity. In (c, d) SuperTOPFLASH (STF) activity was measured as a positive control for Wnt/β-catenin pathway activation. RLU: relative luciferase units. In (b, c) Data represent mean ± s.e.m., *P < 0.05, one-way ANOVA and Kruskal–Wallis post hoc test. In (d) Data represent mean ± s.e.m., **P < 0.01, two-tailed Mann–Whitney test. ns not significant

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