Fig. 1: Profiling of NSC derived hiPSC obtained from Ctrl and SZP.

Immunostaining for Ctrl (a, c, and e) and SZP (b, d, and f) NSC specific markers; (a and b) Nestin (green) and nuclear marker DAPI (blue), (c and d) Pax6 (red) and corresponding nuclear marker DAPI (e and f); Scale bar = 100 um. Representative images from N = 3 (3 Ctrl NSC and 3 SZP NSC). g Percentage of positive cells, with 98% (±0.003) and 97% (±0.01) Nestin positive cells for Ctrl and SZP NSCs respectively; 99% (±0.002) and 99.7% (±0.002) Pax6 positive cells for Ctrl and SZP NSCs respectively. No Oct4 positive cells were detected in NSC of Ctrl and SZP. Data is shown as mean ± SD from N = 3. h Cell division was monitored for 12 h and no significant differences in proliferation were found between Ctrl and SZP NSC. Data for each cell line (Ctrl #1, #2, #3 and SZP #1, #2, #3) is shown in a correlative order control and graphed as mean ± SD. i, j Morphological parameters such as cell area (i) and cell roundness (j) did not show detectable differences among Ctrl and SZP groups. Data for each cell line (Ctrl #1, #2, #3 and SZP #1, #2, #3) is shown in a correlative order control and graphed as mean ± SD