Fig. 2: SZP NSC present an imbalance in neuro-angiogenic factor secretion.
From: hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis
a Representative pictures of angiogenic profile secretome from Ctrl NSC and SZP NSC. Red boxes indicate positive internal control spots; blue boxes indicate negative controls. b Quantification of angiogenic protein levels of (a) 3 Ctrl NSC CM versus 3 SZP NSC CM. Data are shown as level of expression relative to internal control and graphed as mean ± SD; *p < 0.05 according to Mann–Whitney test. c Heatmap depicting the levels of angiogenic proteins present in 3 Ctrl NSC CM (#1, #2 and #3), 3 SZP NSC CM (#1, #2 and #3), 3 Ctrl Nsp CM (#1, #2 and #3) and 3 SZP Nsp CM (#1, #2 and #3). Data are shown as average level relative to internal control. d–g Quantification of IGBP-2 (d), VEGFA (e), TIMP-1 (f), and NRG1-B1 (g), in each of the CM. Data for each cell line (Ctrl #1, #2, #3 and SZP #1, #2, #3) is shown in a correlative order. Values are shown as the level of protein expression relative to internal control and graphed as mean ± SD; *p < 0.05, **p ≤ 0.01 according to Mann–Whitney test. h–m Analysis of non-canonic neuroangiogenic factors expression by qPCR in 3 Ctrl NSC and 3 SZP NSC. Data for each cell line (Ctrl #1, #2, #3 and SZP #1, #2, #3) is shown in a correlative order. Data are shown as mean ± SD; *p < 0.05 according to Mann–Whitney test. (n) Expression of SEMA3A was evaluated in 3 Ctrl and 3 SZP NSC CM. Data for each cell line (Ctrl #1, #2, #3 and SZP #1, #2, #3) is shown in a correlative order. (o) Quantification of protein bands of (n) reveal increased SEMA3A expression in SZP NSC. Data are shown as mean ± SD; *p < 0.05 according to Mann–Whitney test
