Fig. 3: SZP NSC CM induce less angiogenesis and migration in vitro than Ctrl NSC CM.
From: hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis
a Cartoon representing the experimental design of the tube formation assay. After 48 h, CM was collected from NSC cultures and applied on HUVEC seeded on matrigel coated wells. After 4 h, tubes (closed polygons) and sprouts were counted. b–d Representative images of tube formation assay when incubating HUVEC on Neural Expansion Media (NEM, used as negative control (b), Ctrl NSC CM (c) or SZP NSC CM (d); Scale bar = 30 µm. e–f Quantification of average number of sprouts (e) or tubes (f) formed in each condition. Data for each cell line (Ctrl #1,#2, #3 and SZP #1, #2, #3) is shown in a correlative order control and graphed as mean ± SD with *p < 0.05 according to Kruskal-Wallis test. g–n Ctrl NSC CM was incubated with 100 µg/ml of bevacizumab (Bvz) to inhibit VEGFA signaling. g–l Representative images of tube formation assay when incubating HUVEC on NEM (g), NEM plus 50 ng/ml VEGFA (h), NEM plus 50 ng/ml VEGFA with 100 µg/ml of bevacizumab (Bvz) inhibitor (i), Ctrl NSC CM (j), Ctrl NSC CM with 100 µg/ml Bvz (k) or SZP NSC CM (l). m–n Quantification of average number of sprouts (m) or tubes (n) formed in each condition. Data is shown as mean ± SD with *p < 0.05, **p ≤ 0.01 and ***p ≤ 0.001 according to Kruskal–Wallis test
