Fig. 2: Loss of directed migration trajectory in isogenic RELN-del neurons.
From: Single-cell trajectory analysis of human homogenous neurons carrying a rare RELN variant
a Plots of 10-cell trajectories emanating from the origin. 1 pixel = 3.42 μm. b Quantification of total migration distance over 4 h (48–52 h after plating). Bars represent the mean ± SD. ***p < 0.001. n = 10. c Upper: Definition of directionality ratio. Lower: Directionality ratio over elapsed time. Plots represent the mean ± SEM. n = 10. d Directionality ratio of the last point trajectory. Bars represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. n = 10. e Schematic diagram showing the cell movement angle at each time point n relative to the coordinate axis. αCelln = angle for Celln. f Distribution of the cell movement angles for each genotype plotted on circular histograms. Each histogram shows a representative cell for each genotype. The diameter represents the cell numbers. g Summary of 10 cells from each genotype shown as a histogram. h The distribution of cells based on their movement angles. Angles are absolute values relative to the origin (negative is clockwise). For example, 0°−20° include both αCelln = 0°−20° and −20°–0°. Each bar represents the proportion of the total analysis points according to the angular movement range 0°–20°, 20°–60°, and 60°–180° for each genotype. CON1 n = 149, CON2 n = 163, IgCON1(+/−) n = 159, IgCON1(−/−) n = 159, IgCON2(+/−) n = 145, IgCON2(−/−) n = 152. *p < 0.05, ***p < 0.001. i Graphical abstract
